A comparative study of Kalonji (Nigella sativa) extracts and oil for antibacterial properties.
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CONTRIBUTORS:
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CONFERENCE NAME:
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CONF. LOCATION:
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Lucknow, India
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CONFERENCE YEAR:
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2003
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PUB TYPE:
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Conference Presentation
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SUBJECT(S):
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Pharmacology, Ethnomedicine, Microbiology
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DISCIPLINE:
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Plant Sciences
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HTTP:
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LANGUAGE:
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English
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PUB ID:
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103-416-777
(Last edited on
2005/06/18 02:07:31 GMT-6)
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SPONSOR(S):
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ABSTRACT:
Abstract
Objective : To screen Kalonji (N. sativa) for antibacterial properties and their distribution in various fractions.
Methods : Kalonji, procured locally was crushed into fine powder and subjected to Soxhlet extraction using methanol, petroleum ether and water to obtain methanolic, ether and aqueous extracts respectively. These were tested in different dilutions against standard strains of Staphylococcus aureus, Pseudomonas aerugenosa and E. coli (NCTC and ATCC strains) using disc-agar diffusion technique on Muellar-Hinton agar plates under standard laboratory conditions. The results were compared with those obtained with pure Kalonji oil (Mohammadia products, Hyderabad).
Results : Methanolic extract exhibited significant inhibition against Staph. aureus and Pseudomonas whereas ether extract did not show any activity against any one. The aqueous extract showed inhibitory activity against all the three bacteria only in 100 mg/disc concentration but aqueous extract obtained after soaking the crushed seeds in water did not show any activity. The Kalonji oil showed activity against standard strains of Staph. aureus and Pseudomonas up to a dilution of 1 : 50 and 1 : 100 respectively.
Conclusion : The antibacterial property of Kalonji appears to be present in the methanolic fraction of Kalonji / Kalonji oil.
e-mail : tsalman@rediffmail.com
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